Specialist – Molecular & Cell Biology

University of California, Berkeley

Associate (Step I-IV)/Full (Step I-V) Specialist – Molecular & Cell Biology – Reducing Immunogenicity of CRISPR-based editing sy

Job #JPF02082

Recruitment Period

Next review date:March 11th, 2019 Apply by this date to ensure full consideration by the committee. Final date: April 5th, 2019 Applications will continue to be accepted until this date, but those received after the review date will only be considered if the position has not yet been filled.


Associate (Step I-IV)/Full (Step I-V) Specialist – Molecular & Cell Biology – Reducing Immunogenicity of CRISPR-based editing systems – Barton Lab

The UC Berkeley Department of Molecular and Cell Biology is seeking to hire a specialist in the Barton Lab to assist with ongoing research on immunity generated against Cas9 and other CRISPR-based gene editing platforms. This position will seek to reduce the immunogenicity of gene editing enzymes, first with proof-of-principle studies in animal models followed by eventual extension into human systems. MINIMUM/BASIC QUALIFICATIONS:

ADDITIONAL QUALIFICATIONS (BY START DATE): • Five (5) years of research experience.

PREFERRED QUALIFICATIONS (BY START DATE): • Demonstrated ability to work independently, plan experimental design, carry out experiments, perform initial interpretation of results, troubleshoot technical issues • Ability to perform basic molecular biology (e.g., PCR, DNA/RNA isolation, subcloning, etc) • Experience with multiparameter flow cytometry (preferably at least 10 parameters), sample preparation, acquisition, and analysis. Familiarity with BD Fortessa analyzers, FACSAria Fusion sorter, and FlowJo analysis software • Experience using mice for experimental analyses, including general husbandry, manipulation, injections, and necropsy. • Experience with general immunological assays, including cell culture of immune cells, measurement of cytokines produced by T cells (ELISA, ELISpot, Luminex, Cytometric bead array), cell proliferation. • Experience quantifying CD8 and CD4 T cell responses to specific antigens in mice, both by ELISpot and flow cytometry. • Experience working with human samples • Analysis of human immune responses (using same metrics/assays as listed above for animal work)

APPOINTMENT This position reports to Dr. Gregory Barton. The initial appointment will be full-time at 100% time for up to two (2) years with the possibility of extension based on satisfactory performance and availability of funding. The approximate start date of this position will be in May 2019.

SALARY AND BENEFITS $56,400– $111,600 at 100% time, depending on qualifications. This position provides full benefits. Salary will be commensurate with qualifications and experience and based on UC Berkeley specialist series salary scale.

TO APPLY Interested individuals should submit application documents as PDFs and names with contact information for at least 3-5 individuals who have agreed to provide a reference for this specific position and a cover letter. (Letters of reference may be requested of the finalists).

Specific questions regarding the recruitment can be directed to Jose Padilla, HR Partner, josepadilla@berkeley.edu .

This recruitment will remain open until filled.

All letters will be treated as confidential per University of California policy and California state law. Please refer potential referees, including when letters are provided via a third party (i.e. dossier service or career center) to the UC Berkeley Statement of Confidentiality (http://apo.berkeley.edu/evalltr.html) prior to submitting their letters. The University of California is an Equal Opportunity/Affirmative Action Employer. All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, sexual orientation, gender identity, national origin, disability, age or protected veteran status. For the complete University of California nondiscrimination and affirmative action policy see: http://policy.ucop.edu/doc/4000376/NondiscrimAffirmAct

The Department is interested in candidates who will contribute to diversity and equal opportunity in higher education through their research.

The University of California, Berkeley has an excellent benefits package as well as a number of policies and programs in place to support employees as they balance work and family.

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  • Cover Letter

  • Statement of Research (Optional)

  • Statement on Contributions to Advancing Diversity, Equity, and Inclusion - Statement on your contributions to diversity, equity, and inclusion, including information about your understanding of these topics, your record of activities to date, and your specific plans and goals for advancing equity and inclusion if hired at Berkeley (for additional information go to https://ofew.berkeley.edu/recruitment/contributions-diversity). (Optional)

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Research assistant / technician


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Research assistant / technician

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