Techniques to be performed include PCR real time, Nucleic Acid Extraction and Purification, sub-cloning of plasmid vectors, DNA and protein electrophoresis, Western blotting, standard biochemical assays (ELISAs, extracellular matrix quantification), histology, and small animal surgery. Prior rodent husbandry and surgery experience is preferred. The applicant will learn how features of a gene circuit affect transduced cell functions critical to matrix generation and tissue repair, in both diseased primary cells (chondrocytes from osteoarthritic patients) and stem cells.
Ph.D. in Biomedical Engineering, Life Sciences, or related fields. Applicants with the skills in designing and performing mammalian cell culture experiments and tissue engineering techniques, as well as extensive experience in molecular biology, and small animal handling are preferred.
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Faculty / researcher
Ann Arbor , United States