The Department of Molecular, Cell, and Developmental Biology (https://mcd.ucsc.edu/) at the University of California, Santa Cruz (UCSC) invites applications for an entry-level research specialist who will work under the direction of Professor Manuel Ares in the Center for Molecular Biology of RNA (RNAC). The selected candidate will participate in research on the structure and function of the splicing apparatus and provide technical support for researchers. Duties will include preparation of reagents such as plasmids, purified proteins, functional RNAs, and active splicing extracts using basic biochemistry and molecular biology skills, including Gibson assembly, bacterial transformation, plasmid purification, and gel electrophoresis. The research specialist will also carry out in vitro splicing and splicing complex formation assays using radiolabeled splicing substrates and partially purified cell extracts. The position will require the incumbent to successfully follow both verbal and written directions, keep organized and accurate records, and employ conscientiousness and dependability.
Applicants with any of the following preferred qualifications are strongly encouraged to apply: Expertise in operating liquid chromatography systems; experience with ultracentrifuges, UV-Vis spectrophotometer, and high-voltage gel electrophoresis; experience with glassware washing and tagging chemical waste for disposal; experience with DNA and RNA extraction/manipulation; and/or experience with culture and transfection of yeast and mammalian cells, including with CRISPR/Cas9 editing.
Researcher / analyst
1156 High St, Santa Cruz, CA 95064
Santa Cruz , United States