Research assistant / technician
The Department of Pediatrics is one of the largest departments within the School of Medicine with approximately 171 Faculty, 50 post-doctoral fellows (both MDs and PhDs) along with over 300 support staff (not including hospital staff). In addition, the Department has 57 clinical residents and fellows distributed across the Divisions. The missions of research, education and patient care are intertwined, and are integral to the goals of the department.
The Division of Host-Microbe Systems & Therapeutics brings together a diverse group of scientists and physicians for interdisciplinary research on the interactions of humans and the microbial world in both health and disease. Fundamental principles of microbiology, immunology, pharmacology, -OMICs (genomics, proteomics, metabolomics, and the human microbiome) and state-of-the-art systems biology are focused on the pathogenesis of common childhood infectious diseases and inflammatory disorders, to inspire innovative discovery programs and translational studies of new drugs and interventions to restore optimal health in childhood and throughout life.
This Lab Assistant position will work in an established laboratory investigating host-pathogen interactions. The research is focused on identifying mechanisms of gut pathogen virulence, with strong interests in immunology and microbiology. Under supervision, the Lab Assistant will assist in performing research exploring virulence of pathogenic bacteria and analysis of the immune response in vitro and in vivo, including models of colitis. This work involves standard immunology, microbiology, molecular biology, and mouse models of disease. In addition, the position includes routine laboratory maintenance and support, such as assisting with general laboratory maintenance, preparation of general laboratory stocks and supplies, and overseeing animal colonies.
Theoretical knowledge in microbiology, immunology, and molecular biology techniques and/or relevant lab experience.
Knowledge of or experience with sterile/aseptic technique.
Knowledge of or experience in gel electrophoresis and transfers, various gel and membrane stains, western blots, analysis of resulting data.
Demonstrated ability to follow written and verbal protocols, techniques, and instructions. Demonstrated organizational and troubleshooting skills, cooperative, enthusiastic and team-oriented, attention to detail and thoroughness in record keeping, ability to take initiative in maintaining and improving lab operations.
Ability to use basic laboratory equipment including centrifuges, spectrophotometers, balances, pH meters, electrophoresis equipment, fume hoods, and biosafety cabinets.
Knowledge of bio and chemical safety techniques and practices. Demonstrated ability to follow established Safety and Biohazardous Material Guidelines and implement measures to ensure compliance and maintain associated records.
Experience with the operation of a personal computer and software applications, such as Microsoft Office (Word, Excel, Access), Photoshop, Acrobat.
Ability to perform basic PCR, ELISA and cloning techniques.
Knowledge of or experience with mouse genotyping.